Fig. 5.

In vivo protein–protein interaction of XSP10, SlSAMT, and stress associated proteins in tomato protoplasts under confocal microscopy. A. Tomato protoplasts co-transformed with split-YFP constructs of SlSAMT and XSP10 proteins showed clear signals in the cytoplasm. B Strong positive interaction of XSP10 with SlTRAX at cytoplasm. C Moderate interaction of SlSAMT with SlCCOAOMT at cytoplasm. D Weak interaction of SlSAMT with SlTRAX. E Weak interaction of XSP10 with SlCCOAOMT. F Tomato protoplasts co-transformed with empty plasmid used as a negative control for split-YFP fusions which did not show any YFP signal. DAPI stained cells of each construct which was used as a comparative marker show a clear nuclear signal (a, e, i, m, q, u). Arrows highlight the strong interactions (b, f, j) while DAPI stained cells in the nucleus. Scale bar 8 μm, 10 μm and 25 μm