Fig. 6.

Phenotypic evaluation of wilt susceptible cultivar Arka Vikas (AV) and multiple disease resistant cultivar Arka Abedh (AA) to Fol 1322 infection. Root colonization by Fol at 24 h after inoculation observed using bright-field microscopy (A). The red arrow indicates fungal colonization to the root surface. The intensity of colonization was measured as the area of mycelia against the total area of an image and quantified by Image J software (B). Image quantification was based on gray levels of 8-bit images. The asterisk symbol represents a significant difference (***P < 0.001). Root dip bioassay of 4 –weeks old tomato seedlings of Arka Vikas and Arka Abhed (C) inoculated with Fol 1322 observed at 21 days post inoculation. (D) Disease symptoms were scored by measuring the fresh weight above the cotyledon and (E) disease index (0–4) of independent 20 plants/treatment. Plant weight was subjected to a pairwise comparison Students t-test, whereas disease index (DI) was determined by non-parametric Kruskal–Wallis test (*p < 0.05,**p < 0.01,***p < 0.001) using PRISM.9.0 GraphPad software