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. 2021 Jul 8;2021:9974509. doi: 10.1155/2021/9974509

Table 5.

IC50 values for CDPK7 binding to MHC class I molecules obtained using the IEDBa.

MHC-II alleleb Start-stopc Peptide sequence Percentile rankd Antigenicity
CDPK7 CDPK7
H2-Db 1882-1891 SSPSSLPTPI 0.15 0.4079
143-152 SNLPNLDRYM 0.21 -0.4284
1637-1646 SQLLSTVYYL 0.24 0.9146

H2-Dd 647-656 KGPTKSAMLL 0.18 0.9201
1612-1621 VRGGELFDLI 0.28 0.1770
1483-1492 VGGRQYGFAI 0.64 0.1561

H2-Kb 1721-1730 IMYLLLRGRL 0.55 1.4751
798-807 SSASFSSRGM 1.0 1.3623
1643-1652 VYYLHKCGIV 1.2 0.1373

H2-Kd 1474-1483 CYVELLSEQV 0.79 0.5079
1445-1454 RYYVLVDNML 1.15 0.9079
822-831 GYSASGGMIV 1.3 0.9579

H2-Kk 1555-1564 TNELYAIKVI 0.12 0.2695
1084-1093 SEGPATTPSI 0.75 0.2366
694-703 DVEGIVDKII 1.5 0.1511

H2-Ld 1589-1598 HPNVIYMKEL 3.8 0.2773
1729-1738 RLPFPINQAF 4.2 0.3317
279-288 YEPHPLLARL 4.6 0.1027

aThe immune epitope database (http://tools.iedb.org/mhci/). bH2-Db, H2-Dd, H2-Kb, H2-Kd, H2-Kk, and H2-Ld alleles are mouse MHC class I molecules. cTen amino acids for analysis were used each time. dLow percentile rank = high level binding; high percentile rank = low level binding; IC50 values = percentile rank. ∗ indicates potential antigenic epitopes (threshold = 0.5).