Table 6.
IC50 values for CDPK7 binding to MHC class II molecules obtained using the IEDBa.
MHC-II alleleb | Start-stopc | Peptide sequence | Percentile rankd | Antigenicity |
---|---|---|---|---|
CDPK7 | CDPK7 | |||
H2-IAb | 1109-1123 | AAGAAAAAATAAAAA∗ | 0.07 | 0.8045 |
1108-1122 | AAAGAAAAAATAAAA∗ | 0.08 | 0.8354 | |
1110-1124 | AGAAAAAATAAAAAF∗ | 0.08 | 0.7176 | |
| ||||
H2-IAd | 1035-1049 | SETQPAMASVASGSS∗ | 0.13 | 0.6766 |
1034-1048 | GSETQPAMASVASGS∗ | 0.15 | 0.7059 | |
1036-1050 | ETQPAMASVASGSSP∗ | 0.25 | 0.6536 | |
| ||||
H2-IEd | 1451-1465 | DNMLYYYRRKGDAKP∗ | 0.14 | 0.6972 |
1452-1466 | NMLYYYRRKGDAKPR∗ | 0.14 | 0.8298 | |
1450-1464 | VDNMLYYYRRKGDAK∗ | 0.19 | 0.6159 |
aThe immune epitope database (http://tools.immuneepitope.org/mhcii). bH2-IAb, H2-IAd, and H2-IEd alleles are mouse MHC class II molecules. cFifteen amino acids for analysis were used each time. dLow percentile rank = high level binding; high percentile rank = low level binding; IC50 values = percentile rank. ∗ indicates potential antigenic epitopes (threshold = 0.5).