Fig. 1. Primary screening identified hits with protection activity against 2-E-induced cell death.
a Schematic of cell based high throughput screening assay. b Overview of first round result. All compounds (4376) and their corresponding activity are represented by circle. The cell viability ratio of Adrug/Amodel displayed along the vertical axis. Top five to eight compounds with a ratio greater than 1 in each plate were considered as effective inhibitors and were displayed in red circle. Data represent the mean of the duplicate results. c Cellular protective ratio of secondary round screening (170 hits). Data represent the mean of the six repeats. Amodel, the absorbance of 2-E transfected cells with DMSO treatment group. Adrug, the absorbance of 2-E transfected cells with 10 μM drug treatment group. d Cell viability ratio of 170 positive compounds. Data represent the mean of the six repeats. Adrug, the absorbance of 10 μM drug treatment group. Amock, the absorbance of DMSO treatment group.