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. 2021 Jul 22;43(4):781–787. doi: 10.1038/s41401-021-00732-2

Fig. 2. Characters of 34 hits on anti-viral activity and 2-E channel inhibition ability.

Fig. 2

a Anti-SARS-CoV-2 efficiency of 34 hits at 10 μM. Vero E6 cells were pre-treated with compounds for 1 h, and then infected with SARS-CoV-2(MOI = 0.01). Viral copies were detected through qRT-PCR. b Purification of full-length 2-E protein with Ni-NTA affinity chromatography. 15% SDS-PAGE gel with coomassie blue staining. Triangle, 2-E proteins [27]. c Schematic of planar lipid bilayer system. d Representative single-channel traces after 2-E exposed to the indicated compounds at 100 μM. Once ion channel conductance was detected, compounds were added to the trans side while stirring to facilitate binding of the compound to the channel. The black arrow indicates the application of compounds (n ≥ 3). “C” means channel close; “O” means channel open.