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Example of the tracings for the calculation of the vascular area and the number of vessels in the airway lamina propria. A white line was firstly used to calculate basement membrane length (black arrows), and secondly, it was used to outline the extracellular matrix area (red arrows). Luminal circumference of each vessel included in a region of interest is outlined in red (vascular area). Each vessel was manually assigned a number for vessel count. Scale bar 100 μm
