Fig. 3.

HCP protects against lethal infection in K18-Ace2 mice. (a) K18-Ace2 transgenic mice and C57BL/6 mice (Day 0–3 n=10 per group; Day 6+, n=7) were infected with SARS-CoV-2 by the IN route. Survival and weight loss were monitored for 17 days and plotted using Prism software. Survival significance determined by log-rank; ***P=0.0001, weight loss significance at the indicated time points was determined by t-test; *P<0.005. (b) Pharyngeal swabs qPCR titres were taken on the indicated time points. Line shows geometric mean titres graphed as copies ml⁻¹. Significance was determined by t-test and denoted by asterisks with indicated p value. (c) Lung qPCR titres were examined on day 6 (n=3 mice per group) or at the time of euthanasia by qRT-PCR (PBS-treated mice n=7 mice). Mean titres+/-SEM of the genome copies ml⁻¹ were graphed. Significance was determined by t-test. NS=not significant.