Table 3. 16S rRNA Oligonucleotide Probes Used in the Present Study.

probe code	specificity	sequence (5′ to 3′)	temperature condition (°C)
			hybridization	washing
Chis150	most of the bacteria in the Clostridium histolyticum (Clostridium clusters I and II)	TTATGCGGTATTAATCTYCCTTT	50	50	Franks et al.143
Lab158	Lactobacillus-Enterococcus group	GGTATTAGCAYCTGTTTCCA	50	50	Harmsen et al.144
Erec482	most of the bacteria in the Clostridium coccoides-Eubacterium rectale group (Clostridium clusters XIVa and XIVb)	GCTTCTTAGTCARGTACCG	50	50	Franks et al.143
Bif164	Bifidobacterium spp.	CATCCGGCATTACCACCC	50	50	Langendijk et al.145
Bac303	most Bacteroidaceae and Prevotellaceae, some Porphyromonadaceae	CCAATGTGGGGGACCTT	46	48	Manz et al.146
Ato291	Atopobium spp.	GGTCGGTCTCTCAACCC	50	50	Harmsen et al.147
Prop853	Clostridium cluster XI	ATTGCGTTAACTCCGGCAC	50	50	Walker et al.148
Fpra655	Faecalibacterium prausnitzii	CGCCTACCTCTGCACTAC	58	58	Hold et al.149
EUB338a		GCTGCCTCCCGTAGGAGT
EUB338IIa	total bacteria	GCAGCCACCCGTAGGTGT	46	48	Daims et al.142
EUB338IIIa		GCTGCCACCCGTAGGTGT

^aThese probes are used together in equimolar concentrations (all at 50 ng μL^–1).