Table 2. Ten Most Abundant Proteins Identified in Buchnera by Their Number of Spectraa.
| protein name | hypothetical function | accession number | theoretical mass (Da) | peptide (number of spectra) |
|---|---|---|---|---|
| molecular chaperone GroEL | chaperone | NP_239860.1 | 57 912 | 52 (192, 80, 223, 21, 117, 51, 216, 57) |
| molecular chaperone DnaK | chaperone | NP_239985.1 | 70 045 | 34 (30, 20, 40, 7, 17, 15, 43, 22) |
| elongation factor Tu | translation | NP_240333.2 | 43 466 | 32 (32, 10, 25, 4, 29, 18, 44, 12) |
| co-chaperonin GroES | chaperone | NP_239859.1 | 10 244 | 11 (37, 8, 45, 2, 20, 8, 24, 10) |
| G-aldehyde 3-phosphate d- hydrogenase A | energy metabolism (glycolysis) | NP_240121.1 | 36 949 | 18 (6, 17, 14, 4, 3, 17, 7, 17) |
| OmpF-like porin | porin, diffusion of low-molecular-weight compounds | NP_240177.1 | 42 701 | 17 (12, 29, 12, 7, 1, 6, 0, 13) |
| alkyl hydroperoxide reductase | scavenger of endogenous hydrogen peroxide | NP_240013.1 | 22 363 | 13 (10, 11, 12, 5, 2, 8, 8, 20) |
| OmpA protein | porin | NP_240151.1 | 39 306 | 11 (10, 17, 8, 6, 1, 8, 3, 11) |
| ketol-acid reductoisomerase | amino acids biosynthesis | NP_240398.1 | 55 828 | 21 (7, 6, 8, 7, 0, 10, 5, 20) |
| 5-methyltetrahydropteroyltriglutamate | amino acids biosynthesis | NP_239871.1 | 87 916 | 18 (23, 2, 19, 0, 5, 4, 7, 2) |
a
The most abundant proteins identified in our MS lists are shown for the full extract (supernatant + pellet) of Buchnera in the combined orange and green aphids. Numbers refer to the peptides found in the two separate determinations, and the values in parentheses correspond to the number of spectra in orange (supernatant, pellet) and green (supernatant, pellet) samples for experiment 1 and orange (supernatant, pellet) and green (supernatant, pellet) samples for experiment 2.