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. 2021 May 18;8:1054–1066. doi: 10.1016/j.toxrep.2021.05.007

Fig. 6.

Fig. 6

Confocal Micrographs of ethanol treated cells. Figure depicts cellular morphology upon exposure to different concentration of ethanol. a1, a2: Control; b1, b2: 0.01 % (v/v); c1, c2: 0.1 % (v/v); d1, d2: 1 % (v/v); e1, e2: 3 % (v/v); f: 7 % (v/v); g: 10 % (v/v) ethanol. Increase in ethanol concentration leads to actin disorganization and membrane instability. Loss of actin can be seen in e1, f and g. Green: FITC-Phalloidin staining actin filaments; Blue: DAPI staining nucleus; images taken at 63 × (oil) objective, NA: 1.40; Scale bar: 20 μm.