Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapiens) |
Human kidney cells (Hek293T) | AATC | CRL-3216 RRID:CVCL_0063 |
Authentication was from AATC. We did not independently authenticate the cell line. |
| Cell line (M. auratus) |
Baby Hamster Kidney cells (BHK-21) | AATC | [C-13] CCL-10 RRID:CVCL_1915 |
Authentication was from AATC. We did not independently authenticate the cell line. |
| Cell line (H. sapiens) |
Neuroglioma (H4) | A gift from Dr Helen Scott and Professor James Uney | Authenticated (STR profiling) and mycoplasma tested (absent) (Eurofins) | |
| Biological sample (R. norvegicus) |
Primary hippocampal and cortical neurons | University of Bristol Animal Services Unit | ||
| Antibody | ‘(mouse monoclonal)’ β–actin | Sigma-Aldrich | A1978 RRID:AB_476692 |
WB ‘(1:1000)’ |
| Antibody | ‘(mouse monoclonal)’ EEA1 | BD Biosciences | 610457 RRID:AB_397830 |
IF ‘(1:250)’ |
| Antibody | ‘(mouse monoclonal)’ FLAG | Sigma-Aldrich | F1804 RRID:AB_262044 |
WB ‘(1:1000)’ |
| Antibody | ‘(mouse monoclonal)’ GFP | Roche | 11814460001 RRID:AB_390913 |
WB ‘(1:1000)’ |
| Antibody | ‘(rabbit polyclonal)’ GluA1 | Merck Millipore | ab1504 RRID:AB_2113602 |
WB ‘(1:1000)’ |
| Antibody | ‘(mouse monoclonal)’ GluA1 | Merck Millipore | MAB2263 RRID:AB_11212678 |
IF ‘(1:100)’ |
| Antibody | ‘(mouse monoclonal)’ GluA2 | Merck Millipore | MAB397 RRID:AB_2113875 |
WB ‘(1:1000)’ IF ‘(1:70)’ |
| Antibody | ‘(rabbit polyclonal)’ mCherry | Abcam | ab167453 RRID:AB_2571870 |
WB ‘(1:1000)’ IF ‘(1:100)’ |
| Antibody | ‘(rabbit polyclonal)’ LRFN2 | Atlas | HPA07660 | WB ‘(1:500)’ |
| Antibody | ‘(mouse monoclonal)’ SNX27 | Abcam | ab77799 RRID:AB_10673818 |
WB for human SNX27 ‘(1:500)’ |
| Antibody | ‘(rabbit polyclonal)’ SNX27 | A kind gift from Dr Martin Playford, NIH, U.S.A | WB for rat SNX27 ‘(1:500)’ |
|
| Antibody | ‘(rabbit polyclonal)’ LAMP1 | Abcam | ab24170 RRID:AB_775978 |
IF ‘(1:200)’ |
| Antibody | ‘(mouse monoclonal)’ LAMP2 | Hybridoma bank | H4B4 RRID:AB_2134755 |
IF ‘(1:500)’ |
| Antibody | ‘(mouse monoclonal)’ Transferrin | Santa Cruz | Sc-65882 RRID:AB_1120670 |
WB ‘(1:1000)’ |
| Antibody | ‘(rabbit polyclonal)’ VPS35 | Abcam | ab97545 RRID:AB_10696107 |
IF ‘(1:200)’ |
| Antibody | ‘(rabbit)’ Alexa Fluor 405 | Invitrogen | A31556 RRID:AB_221605 |
IF ‘(1:200–400)’ |
| Antibody | ‘(mouse)’ Alexa Fluor 488 | Invitrogen | A21202 RRID:AB_141607 |
IF ‘(1:400)’ |
| Antibody | ‘(rabbit)’ Alexa Fluor 568 | Invitrogen | A10042 RRID:AB_2534017 |
IF ‘(1:400)’ |
| Antibody | ‘(mouse)’ Alexa Fluor 568 | Invitrogen | A10037 RRID:AB_2534013 |
IF ‘(1:400)’ |
| Antibody | ‘(rabbit)’ Alexa Fluor 647 | Invitrogen |
A31573 RRID:AB_2536183 |
IF ‘(1:400)’ |
| Antibody | ‘(mouse)’ Alexa Fluor 647 | Invitrogen | A31571 RRID:AB_162542 |
IF ‘(1:400)’ |
| Antibody | ‘(mouse)’ Alexa Fluor 680 | Invitrogen | A21057 RRID:AB_141436 |
WB ‘(1:10,000)’ |
| Antibody | ‘(rabbit)’ Alexa Fluor 800 | Invitrogen | SA535571 RRID:AB_2556775 |
WB ‘(1:10,000)’ |
| Peptide, recombinant protein | LRFN2 peptides | Genscript | ||
| Chemical compound, drug | Sulpho NHS-SS-Biotin | Thermo Fisher | 21331 | |
| Chemical compound, drug | Streptavidin beads | GE Healthcare | 17-5113-01 | |
| Chemical compound, drug | GFP/RFP-Trap beads | ChromoTek | gta-20, rta-20 | |
| Recombinant DNA reagent | pEGFP-C3-GLUA1(CT) | This paper | available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pEGFP-C3-GLUA2(CT) | This paper | available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pEGFP-C3-GLUA3(CT) | This paper | available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pEGFP-C3-GLUA4(CT) | This paper | available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pEGFP-C1-LRFN1(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN2(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN3(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN4(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN5(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-SLC1A3(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-SLC4A7(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN1ΔPDZbm(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN2ΔPDZbm(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN4ΔPDZbm(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1- SLC1A3ΔPDZbm(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1- SLC4A7ΔPDZbm(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN2(pE786A)(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pEGFP-C1-LRFN2(pV789A)(CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pcDNA3.1-FLAG-SNX27 | This paper | available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pCMV2-SEP-GluA1 | Addgene | 64942 RRID:Addgene_64942 |
Blanco-Suarez and Hanley, 2014 |
| Recombinant DNA reagent | pcDNA3-SEP-GluA2 | Addgene | 64941 RRID:Addgene_64941 |
Ashby et al., 2004 |
| Recombinant DNA reagent | pCMV2-myc-GluA2 |
Leuschner and Hoch, 1999
available on request from Kevin Wilkinson |
||
| Recombinant DNA reagent | pmCherryC1-LRFN2(FL) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pmCherryC1-LRFN2(FN/TM/CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pmCherryC1-LRFN2(TM/CT) | This paper | available on request from Peter Cullen | |
| Recombinant DNA reagent | pXLG3-GFP-H1-SNX27 shRNA (Rat) (+/- WPRE) |
Binda et al., 2019 | Rat target sequence 5’-aagaacagcaccacagaccaa-3’ available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pXLG3-GFP-H1-SNX27 shRNA (Human) (+/- WPRE) |
This paper | Human target sequence 5’- aagaacagtactacagaccaa-3’ available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pXLG3-GFP-H1-LRFN2 shRNA (+/- WPRE) |
This paper | target sequence 5’-acgacgaggtactgattta-3’ available on request from Peter Cullen | |
| Recombinant DNA reagent | pXLG3-GFP-H1-control shRNA (+/-WPRE) |
Binda et al., 2019 | non-targeting sequence 5’-aattctccgaacgtgtcac-3’ available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pSinRep5-GFP/ pSinRep5-GFP-SNX27 | This paper | available on request from Kevin Wilkinson | |
| Recombinant DNA reagent | pSinrep-mcherry/ pSinRep5-mCherry-LRFN2 | This paper | available on request from Peter Cullen |