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. 2021 Jul 22;19:143. doi: 10.1186/s12915-021-01079-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4. — Transitioning to early flowering in the high-elevation groups. a The Fst values between HG and LG in the genome region (Chr1:69,000–69,800 kb) which includes the FLC gene. b The FLC transcripts in the Kunming (KM) accession and corresponding gene model. The former fifth intron was transcribed (pale blue) and resulted in a new long transcript formed from the fifth to the sixth exons (blue). c Nucleic acid and amino acid sequence alignments of the fifth exon and partial fifth intron of the FLC gene from pennycress as well as A. thaliana and E. salsugineum. The mutations from “GT” to “CT” in the fifth intron are highlighted in red. HG, high-elevation group; MG, middle-elevation group; LG, low-elevation group; EF, early flowering; LF, late flowering