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. 2021 Jul 8;12:650216. doi: 10.3389/fphar.2021.650216

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Copyright © 2021 Xian, Li, Zhang, Li, Zhu, Li, Tan, Lin, Li and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of GL-pp on TAM polarization and TNF-α serum level in mice with B16-F10-luc-G5 and SF. (A) The flow cytometry results with CD86 and CD206 label to show TAM polarization. The type of tumor-related macrophages in pulmonary metastasis tumor tissue was detected with CD86 for type M1 macrophage and CD206 for type M2 macrophage. (B) The statistical results of M1/M2 ratio. (C) The TNF-α serum level in mice detected by ELISA. n = 5. The results are shown as mean ± SD. **p <0.01 compared with the CON group; ^## p < 0.01 compared with the Tumor group; ^△△ p < 0.01 compared with the T + SF group.