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. 2021 Jul 22;19:79. doi: 10.1186/s12964-021-00761-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Crumbs2 mediates adherens junction disassembly and apical detachment of epiblast cells as they undergo EMT during gastrulation in the mouse embryo. A During gastrulation, wild-type epiblast cells near the primitive streak undergo EMT that involves basement membrane breakdown, apical constriction and basal positioning of the nucleus, and loss of E-cadherin containing adherens junctions (AJs). EMT correlates with downregulation of the transcription factor (TF) Sox2 and upregulation of the EMT-TF Snail1. Cells that successfully undergo EMT detach from the epithelium and migrate into the primitive streak in a process called ingression. B In Crumbs2 mutant (Crb2-/-) embryos, mosiacally labeled epiblast cells undergo apical constriction and basal nuclear displacement, but fail to detach from the epithelium [91]. Crb2-/- cells develop an elongated morphology and remain attached to the epithelium via E-cadherin. Apical Myosin II accumulation is reduced in Crb2-/- epiblast relative to wild-type (not shown), suggesting a link between Crb2 and actomyosin activity during EMT [91]