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. 2021 Jul 22;12:418. doi: 10.1186/s13287-021-02497-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Verification of tRF-5026a expression in tissues, plasma samples, and cells. a Electrophoretogram of tRF-5026a qRT-PCR products from two representative gastric cancer tissues. b T-A clone sequencing results of tRF-5026a qRT-PCR products with the original tRF-5026a sequence shown, the sequence of the two adaptors, and the crossing joint primer sequences. c Expression levels of tRF-5026a in gastric cancer tissues (n = 86). A larger ΔC_q value indicates lower expression. d tRF-5026a (18 nt) and its mature tRNA (76 nt) were detected by Northern blotting. Three representative paired gastric cancer tissues (T) and adjacent noncancerous tissues (N) are shown. The 5S rRNA was used as a loading control. e The expression level of tRF-5026a was significantly downregulated in 79.07% (68/86) of gastric cancer tissues compared with normal tissues. f tRF-5026a levels in plasma samples from gastric cancer patients and healthy controls. A larger ΔC_q value indicates a lower expression. n = 37. g tRF-5026a expression levels in cell lines. Low expression levels of tRF-5026a in gastric cancer cell lines were found compared with the normal gastric mucosal epithelial cell line GES-1. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns, no significance