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. 2021 Jul 22;12:421. doi: 10.1186/s13287-021-02501-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — miR-20a-5p was upregulated during the osteogenesis of hDPSCs. A hDPSCs were positive for CD29 and CD44, while negative for CD34 and CD45. B The morphology of primary hDPSCs was spindle-shaped fibroblast-like. C–E Osteogenic and chondrogenic differentiations were evaluated by ARS and Alcian Blue staining respectively, and Oil Red O staining was performed for testing adipogenic differentiation. F ALP and ARS staining after osteogenic induction for 1, 7, and 14 days. G RUNX2 and OPN mRNA levels after osteogenic induction for 1, 7, and 14 days. H The expression levels of miR-20a-5p from day 1 to day 14. *p <0.05 and **p <0.01 compared with osteogenic induction for 1 day