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. 2021 Jul 22;12:421. doi: 10.1186/s13287-021-02501-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — miR-20a-5p facilitated osteogenic differentiation of hDPSCs in vitro. A Transfection efficiency in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. B The relative expression of miR-20a-5p in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups at day 7. C The relative expression of miR-20a-5p in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups at day 14. D ALP staining in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. E ALP activity in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. F Quantification of ARS in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. G The relative mRNA expression levels of RUNX2, OPN, and BSP in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. H Protein levels of RUNX2, OPN, and BSP in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. I Quantification of protein levels of RUNX2, OPN, and BSP in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. *p <0.05 and **p <0.01 compared with the miR-NC group