Fig. 3.

Hypoxic preconditioning promotes the neuroprotection and cellular survival of OM-MSCs in vivo. A Representative images of Nissl staining in the cortex regions on days 7, 14, and 28 following ICH. Black arrows indicate apoptotic neurons. B Neuronal loss in the cortex and region was significantly ameliorated by OM-MSC treatment, especially the hypoxia OM-MSCs. C, D HE staining micrographs demonstrate the reduced number of eosinophilic cells in the cortex of ICH mice compared with those in saline group after OM-MSC transplantation. Note that the deep blue nuclei surrounded by white vesicles indicate injured cells (eosinophilic cells, indicated by black arrows), and cells with light blue nuclei are the healthy cells. E Representative PCR image of Alu-sx in the brain tissue that received injections of normoxia OM-MSCs, hypoxia OM-MSCs at days 7, 14, and 28 post-ICH. Data are expressed as the mean ± SEM (n = 4) (^*P <0.05, ^**P <0.01, ^***P <0.001, ns. no significance)