Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 22;12:413. doi: 10.1186/s13287-021-02480-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 8 — miR-326 promotes autophagy via the PTBP1/PI3K signaling pathway. A The potential binding sites for miR-326 on the 3′UTR of PTBP1. B 293T cells were co-transfected with miR-326 mimic or miRNA control and with a luciferase reporter vector containing WT or mutant 3′UTR of PTBP1. C The expression level of PTBP1 negatively correlated with the level of miR-326 in OM-MSCs. D Compared with those in Hemin-exposed normoxia OM-MSCs, the expression levels of p-PI3K/PI3K and PTBP1 were robustly decreased in Hemin-exposed hypoxia OM-MSCs. E Overexpressed PTBP1 increased the expression of PTBP1 and p-PI3K/PI3K. F Under miR-326 mimics treatment, overexpressed PTBP1 increased the expression of p21, P53, p16INK4A, and P62 protein and decreased the expression of LC3II/I and Bcelin-1 protein. G Treatment with PI3K activator partially reversed the downregulation of p-PI3K/PI3K and P63 and the upregulation of LC3II/I and Bcelin-1 induced by the miR-326 mimics. Data are expressed as the mean ± SEM (n = 3) (^*P <0.05, ^**P <0.01, ^***P <0.001, ns. no significance)