Figure 3.
The effects of TUSC7 overexpression on the growth, migration and invasion of TNBC cells. (A) The Overexpression of TUSC7 was performed by transfecting MDA-MB-468 cells with the TUSC7-DOX vector (TUSC7 Over). Expression was confirmed by qRT-PCR. (B) CCK8 assays. MDA-MB-231 cells were transfected with TUSC7-DOX (TUSC7 Over) or scramble vector for five days. (C) Cell cycle analysis of MDA-MB-231 cells transfected with TUSC7-DOX (TUSC7 Over) or scramble vector for 5 days. (D and E) Transwell migration and invasion assays of MDA-MB-231 cells transfected with TUSC7-DOX (TUSC7 Over) or scramble vector for 5 days. Magnification, 100X. **P<0.001; ***P<0.0001.