Independently triggered release of three distinct proteins from a single gel. (a) Experimental schematic of a hydrogel with homogenously tethered EGFP-R-N3, mCherry-P-N3, and mCerulean-E-N3. (b) Release for each protein under every relevant environment (EGFP, mCherry, mCerulean from left to right for each treatment condition). Colored bars (green = EGFP, red = mCherry, blue = mCerulean) indicate conditions expected to yield release while unshaded bars indicate conditions not expected to yield release. Error bars correspond to ±1 standard deviation about the mean for n = 3 experimental replicates.