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. Author manuscript; available in PMC: 2021 Jul 22.
Published in final edited form as: J Mater Chem B. 2017 Apr 4;5(23):4435–4442. doi: 10.1039/c6tb03400d

Fig. 4.

Fig. 4

a) Fluorescent proteins are functionalized with ONH-NPPOC through NHS chemistry. b) Off-stoichiometric hydrogels are prepared (1:0.9, CHO:ONH-NPPOC), leaving unreacted benzaldehydes uniformly distributed throughout the polymer network. Subsequent treatment of the gel with a fluorescent protein and photomasked UV irradiation immobilizes the protein in irradiated regions. c) Micron-resolution patterns obtained by spatially immobilized GFP (scale bar = 100 μm). d) 3D z-stack confocal imaging displaying uniform protein concentrations over 100 μm (scale bar = 100 μm). This patterning approach can be applied to e) simultaneously, and f) sequentially, pattern multiple ONH-NPPOC-functionalized biomolecules. e) Simultaneous patterning of GFP and mCherry (top to bottom: GFP, mCherry, and overlay, scale bar = 200 μm). f) Sequential patterning of GFP and mCherry (top to bottom: GFP, mCherry, and overlay, scale bar = 200 μm).