FIGURE 1.

Phenotypic assessment of anticlonogenic and cytotoxic activities of compounds. (A,B) A higher DSS₃ reflects a more potent effect of formyl aminobenzazulenones (A) and aminobenzazulenones (B) tested at a concentration range of 0.6–40 μM on KRAS-mutant MDA-MB-231 and HRAS-mutant Hs 578T 3D spheroid formation in low attachment condition without serum. Data represent mean values ± SD, n ≥ 3. Numbers above the bars indicate the KRAS/HRAS mutant cell line DSS₃ ratios. (C,D) The relative toxicity of formyl aminobenzazulenones (C) and aminobenzazulenones (D) was assessed in the CellTox Green assay. Cells were grown as 2D adherent monolayers overnight and then treated for 72 h with 1 μM OphA or 10 μM of the indicated benzazulenones. Data represent mean values ± SD, n ≥ 2. The statistical significance levels are annotated as *p < 0.05; **p < 0.01; ****p < 0.0001, or ns, not significant.