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Assessment of lysine-dependent CaM-binding activity of OphA, formyl aminobenzazulenone 1, and aminobenzazulenone 8. Time course of lysine-dependent CaM-binding activity of OphA (A), compound 1 (B), and compound 8 (C) as measured in the fluorescence polarization assay using F-CaMKII peptide as the fluorescent probe. OphA displayed negligible binding with mutCaM compared to wtCaM; hence, no IC50 values could be derived (Supplementary Figures 5A,B).
