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. Author manuscript; available in PMC: 2021 Nov 5.
Published in final edited form as: Nature. 2021 May 5;593(7860):602–606. doi: 10.1038/s41586-021-03524-0

Extended Data Fig. 5 |. Imbalanced DGCR8: DROSHA stoichiometry during EB differentiation drives Microprocessor aggregation.

Extended Data Fig. 5 |

a, PCA of the WT and ΔSL1 cells by all expressed mRNAs during mESC-to-EpiLC differentiation. b, c, The relative expression and calculated ratio of DGCR8 and DROSHA mRNA based on RNA-seq data during EB differentiation over a 13-day time course. d, Western blot of DGCR8 and DROSHA at different time points of WT mESC-to-EB differentiation. Density of the DROSHA and DGCR8 bands were normalized to the ACTINB band to calculate the relative protein ratios. e, Western blot of DGCR8 and DROSHA in differentiated EB cells (Day 8) before and after treatment with a protease inhibitor MG132(10uM) for 2 hours. Density of the DROSHA and DGCR8 bands were normalized to the ACTINB band to calculate the relative protein ratios. f, Representative images of Microprocessor aggregates in differentiated EB cells (Day 8) derived from dual-reporter ESCs endogenously expressing both mCherry-DGCR8 and eGFP-DROSHA fusion proteins.