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. Author manuscript; available in PMC: 2021 Jul 22.

Published in final edited form as: Naunyn Schmiedebergs Arch Pharmacol. 2013 Dec 22;387(4):329–339. doi: 10.1007/s00210-013-0950-4

Fig. 2 — a: Fsk-induced gene regulation in AC overexpressing BSMC compared to control BSMC. RT² q PCR arrays (SA Biosciences) were used to measure mRNA levels following 24 h treatment with 1μM Fsk. mRNA levels of AC overexpressing, Fsk-treated cells are expressed as fold change with respect to lacZ (control), Fsk- treated. Amphiregulin (AREG), Secretogranin II (SCG2), Cyclin D1 (CCND1), Interleukin 6 (IL-6), Somatostatin (SST). Data are presented as fold change over lacZ, mean ± SEM, n=3. b: IL-6 protein production in AC2 or AC6 overexpressing BSMC. IL-6 protein in cell culture media was measured by ELISA following 24 h treatment with 1 μM of the indicated drug. Data are presented as % change with respect to lacZ, mean ± SEM, n=3. * indicates p<.05 compared to lacZ

Fig. 2 — a: Fsk-induced gene regulation in AC overexpressing BSMC compared to control BSMC. RT² q PCR arrays (SA Biosciences) were used to measure mRNA levels following 24 h treatment with 1μM Fsk. mRNA levels of AC overexpressing, Fsk-treated cells are expressed as fold change with respect to lacZ (control), Fsk- treated. Amphiregulin (AREG), Secretogranin II (SCG2), Cyclin D1 (CCND1), Interleukin 6 (IL-6), Somatostatin (SST). Data are presented as fold change over lacZ, mean ± SEM, n=3. b: IL-6 protein production in AC2 or AC6 overexpressing BSMC. IL-6 protein in cell culture media was measured by ELISA following 24 h treatment with 1 μM of the indicated drug. Data are presented as % change with respect to lacZ, mean ± SEM, n=3. * indicates p<.05 compared to lacZ