FIG 2.
(A to H) DIC and fluorescence micrographs of MRSA LAC-FChP1GFP cells treated for 3 h with either DMSO vehicle (A to D) or 4 μg/ml (4× MIC) TXA707 (E to H) just prior to visualization. (B to D) Insets depict enlargements of the regions enclosed by the small cyan boxes. (I) Localization of PBP1 (green) and FtsZ (red) is schematically depicted, with the numbered arrows in the scheme reflecting the correspondingly numbered arrows in the florescence micrographs. Scale bars for panels A to H represent 2 μm, while those for the insets in panels Bto D represent 0.5 μm. (J) Bar graph showing the prevalence of the various FtsZ and PBP1 phenotypes observed in both vehicle-treated cells (n = 919) and TXA707-treated cells (n = 535). Each percentage reflects an average of 5 different fields of view, with the number of cells in each field of view ranging from 98 to 256. The indicated error bars reflect the standard deviation from the mean. The statistical significance of differences in the FtsZ and PBP1 phenotypes were analyzed as described in the legend to Fig. 1. n.s., not significant.