FIG 5.
(A to H) DIC and fluorescence micrographs of MRSA LAC-FChP4GFP cells treated as described in the legend for panels A to H in Fig. 2. (I) Localization of PBP4 (green) and FtsZ (red) is schematically depicted, with the numbered arrows in the scheme reflecting the correspondingly numbered arrows in the florescence micrographs. Scale bars for panels A to H represent 2 μm. (J) Bar graph showing the prevalence of the various FtsZ and PBP4 phenotypes observed in both vehicle-treated cells (n = 300) and TXA707-treated cells (n = 326). Each percentage reflects an average of 3 to 5 different fields of view, with the number of cells in each field of view ranging from 56 to 162. The indicated error bars reflect the standard deviation from the mean. The statistical significance of differences in the FtsZ and PBP4 phenotypes were analyzed as described in the legend to Fig. 1. n.s., not significant.