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. 2021 Jul 8;9:715674. doi: 10.3389/fcell.2021.715674

FIGURE 4.

FIGURE 4

The immune components of different clusters were evaluated. (A) The results of SSGSEA showed that the low expression of CD34 and CD276 in the Cluster1 group was the low immune score group, while the high expression of CD34 and CD276 in the Cluster2 group was the high immune score group (*P < 0.05, **P < 0.01, ***P < 0.001). (B) The immune cell composition of Cluster1 and Cluster2 was further analyzed using the Ciborsort R package. The results showed that in Cluster2, the ratio of activated memory T cellsCD4 and dendritic resting cells was lower than that in Cluster1 (*P < 0.05, **P < 0.01), while the ratio of regulatory T cells was relatively higher in Cluster1 (*P < 0.05, **P < 0.01, ***P < 0.001). (C,D) The infiltration level of activated memory T cellsCD4 was negatively correlated with CRC TNM stage, the infiltration level of T cells (Tregs) was positively correlated with tumor TNM stage (*P < 0.05). (E) There was no significant difference in the expression of immune checkpoint PDL1 and SiGLEC15 between the groups with high and low immune scores (P > 0.05); the expression levels of TIGIT and CTLA4 were up-regulated in the group with high immune scores, and the expression levels of HAVCR2 and LAG3 were significantly up-regulated in the group with high immune scores (*P < 0.05, **P < 0.01, ***P < 0.001).