TABLE 1.

Bacterial strains, plasmids, and antibody used in this study.

Strain or plasmid	Relevant characteristicsa	Source
Strains
S. suis
SC19	Virulent strain isolated from the brain of a dead pig; Serotype 2	Laboratory collection
ΔSsPepO	The SsPepO deletion mutant of SC19	Laboratory
CΔSsPepO	Complemented strain of ΔSsPepO; SpcR	Laboratory
E. coli
DH5α	Cloning host for recombinant vector	TaKaRa
BL21 (DE3)	The expression host for pET-30a and their derivative	TransGen
Plasmids
Pet-30a	Expression vector; KanR	Novagen
Pet30a-SsPepO	pET30a containing the SsPepO locus	Laboratory
Antibody
Anti-SsPepO	Anti-SsPepO ployclonal rabit antibody	Laboratory

^aRestriction sites are underlined; Kan^R, Kanamycin resistance; Spc^R, spectinomycin resistance.