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. 2021 Jul 8;12:627394. doi: 10.3389/fphar.2021.627394

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Copyright © 2021 Wu, Xie, Liu, Gao, Qu, Mo, Xu, Chen and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lack of effects on cancer cell growth and on levels of vimentin and major oncoproteins. (A) R491 did not inhibit proliferation of cancer cells A549, AGS, U87, SMMC-7221, HUH7, and PANC-1. (B, D) Semi-quantification of oncoproteins in (B) A549 or (D) PANC-1 cells by the Human XL Oncology. The lysates were prepared from A549 or PANC-1 cells treated with either DMSO or R491 at 5 µM for 48 h. See Supplementary Figure S1 and Supplementary Table S2 for coordinates detail. (C, E) The graph summarizes the relative signal intensity of indicated proteins in (C) A549 or (E) PANC-1 cells.