Skip to main content
. 2021 Jul 8;12:687947. doi: 10.3389/fphys.2021.687947

FIGURE 3.

FIGURE 3

Delayed angiogenesis after acute ischemic stroke in T2DM. (A) Cresyl violet (CV) staining on day 3. NS, non-diabetic sham group (Day 3); N3, non-diabetic cerebral ischemic group (Day 3); DS, diabetic sham group (Day 3); D3, diabetic cerebral ischemic group (Day 3). The unstained area was the infarction area. (B) The volume of cerebral infarction and brain edema was calculated by CV staining on day 3. (C) Garcia score was evaluated on D1–D7 after cerebral ischemia (n = 8 per group). (D) The expression of VEGF-A, Ang-2, Jagged1, Notch1, NICD, and Hes1 in ischemic penumbra was assessed by Western blot (n = 3 per group). (E,F) The expression of VEGF-A and Ang-2 protein were calculated in NDI group and DI group. (G,H) The gene expression of Hif-1a, VEGF-A, Ang-2, Ang-1, TGF-β, and PDGF-β was showed in NDI group and DI group. *p < 0.05 vs. sham group, **p < 0.01 vs. sham group, ***p < 0.001 vs. sham group, ****p < 0.0001 vs. sham group; #p < 0.05 vs. NDI group, ##p < 0.01 vs. NDI group, ###p < 0.001 vs. NDI group, ####p < 0.0001 vs. NDI group.