Figure 3.

Curcumin-induced atheroprotection associates with attenuated Th1 and increased regulatory response. (A, B) Spleen cell suspensions from curcumin- or vehicle-treated mice from Figure 1 were stained with appropriate mAbs to determine the frequency of (A) IFNγ⁺ CD4⁺, TNFα⁺ CD4⁺, and IFNγ⁺ CD8⁺ cells, as well as (B) FOXP3⁺ CD4⁺, IL-10⁺ MHCII^hi CD11c⁺ DC and IL-10⁺ CD19⁺ B cells by flow cytometry. (C) Popliteal lymph node (pLN) cell suspensions were also obtained and the frequency of the FOXP3⁺ CD4⁺ cell population quantified. Left panel shows representative dotplots. Each dot in the right panel represents an individual animal, and the line represents the mean from 8 mice/group. *P < 0.05; **P < 0.01; ***P < 0.001 (Mann-Whitney U test).