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. 2021 Apr 23;1(5):646–659. doi: 10.1021/jacsau.1c00054

Table 2. Kinetic pKa Values for Wild-Type Enzymes and Mutants Obtained from Fits of the pH-Rate Dataa.

enzyme pKa1 pKa2
WT PTP1B 5.08 ± 0.20 6.44 ± 0.26
PTP1B T177G 5.44 ± 0.37 6.37 ± 0.28
WT YopH 5.02 ± 0.27 5.58 ± 0.34
YopH G352T 4.06 ± 0.23 5.70 ± 0.24
a

The pKa1 value arises from the cysteine nucleophile and pKa2 from the aspartic acid.