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. 2021 Jul 8;12:692832. doi: 10.3389/fmicb.2021.692832

FIGURE 7.

FIGURE 7

The addition of exogenous rJagged1 enhances the secretion of pro-inflammatory mediators in RAW264.7 cells against H. pylori infection. RAW264.7 cells were pretreated with rJagged1 (10 ng/ml) for 24 h and then co-cultured with H. pylori (MOI = 50) for 12 h. Human IgG was used as negative control for rJagged1, and PBS was used as control for H. pylori infection. Total RNA of each group was extracted. (A) qPCR was performed to examine the mRNA expression of Hes1. (B) qPCR was performed to examine the mRNA expression of iNOS. (C) Griess was performed to test the production of NO in the supernatant. (D) qPCR was performed to examine the mRNA expression of pro-inflammatory cytokines IL1β, IL6, and TNFα and anti-inflammatory cytokines IL10 and TGFβ. (E) ELISA was performed to examine the protein expression levels of pro-inflammatory cytokines IL1β, IL6, IL12p70, TNFα, and IFNγ and anti-inflammatory cytokines IL10 and TGFβ in the supernatant. β-actin was used as reference gene. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001; N.S. represents no significant difference.