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. 2021 Jul 8;12:692832. doi: 10.3389/fmicb.2021.692832

FIGURE 8.

FIGURE 8

Jagged1 antibody enhances the secretion of pro-inflammatory mediators in RAW264.7 cells against H. pylori infection. RAW264.7 cells were pretreated with 3 μg/ml Jagged1 Ab and then co-cultured with H. pylori (MOI = 50) for 12 h. Mouse IgG was used as control for anti-Jagged1 Ab. PBS was used as control for H. pylori. Total RNA of each group was extracted. (A) qPCR was performed to examine the mRNA expression of Hes1. (B) qPCR was performed to examine mRNA expression of Jagged1. (C) Griess was performed to assess the production of NO in the supernatant. (D) qPCR was performed to examine the mRNA expression of proinflammatory cytokines IL1β, IL6, and TNFα and anti-inflammatory cytokines IL10 and TGFβ. (E) ELISA was performed to examine the protein level of proinflammatory cytokines IL1β, IL6, IL12p70, TNFα, and IFNγ and anti-inflammatory cytokines IL10 and TGFβ in the supernatant. IgG + H. pylori inf: Mouse IgG-pretreated and H. pylori infected group; Jag1 Ab + H. pylori inf.: anti-Jagged1 Ab-pretreated and H. pylori-infected group. β-actin was used as reference gene. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001; N.S. represents no significant difference.