Skip to main content
. 2021 Jul 22;16(7):e0242236. doi: 10.1371/journal.pone.0242236

Fig 1. Transcriptional and translational levels of cortical CSTB in 3-month-old mice.

Fig 1

(A) Levels of Cstb mRNA in the cortex of 3-month old mice. Data are relative to Actb and Gapdh housekeeping genes, and represented as mean expression levels for mice of each genotype ± SEM. Duplication of Cstb caused an increase in the relative levels of Cstb mRNA (F(1,7) = 7.240, p = 0.031). No effect of sex, tgAPP, or interaction between dupCstb and tgAPP was evident (n = 3–5 per genotype, 9 females and 9 males in total). (B) Representative western blot probed with anti-CSTB and anti-β-actin antibodies. (C) Protein band density was quantified using ImageJ, normalised to β-actin, and is shown as fold-change relative to wildtype levels (mean ± SEM), n = 5 for each genotype (9 females and 11 males in total). (D) Representative western blot probed with anti-APP C-Terminal Fragment (CTF) and anti-β-actin antibodies. (E) APP protein band density was quantified using ImageJ, normalised to β-actin, and is shown as fold-change relative to wildtype levels (mean ± SEM), n = 5 for each genotype (9 females and 11 males in total). (F) CTF protein band density was quantified using ImageJ, normalised to β-actin, and is shown as fold-change relative to wildtype levels (mean ± SEM), n = 5 for each genotype (9 females and 11 males in total). Bonferroni post-hoc pair-wise comparisons p < 0.05 = *, p < 0.01 = **, p < .001 = ***, p < 0.0001 = ****.