Table 1. General features, mobile genetic elements (MGEs), antimicrobial resistance associated with MGEs, CWA protein genes, and CRISPR of six representative MRSP isolates.

	Strain-ST (source)
	AH18-ST45 (dog)	VB88-ST45 (human)	AK9-ST112 (dog)	VB16-ST112 (human)	AI14-ST181 (dog)	AP20-ST181 (human)
Features
Genome size (bp)	2,623,199	2,673,267	2,789,057	2,791,609	2,719,425	2,734,754
GC content (%)	37.5	37.5	37.3	37.5	37.5	37.5
CDS	2,427	2,513	2,610	2,655	2,533	2,571
tRNA	59	59	59	59	59	59
tmRNA	1	1	1	1	1	1
miscellaneous RNA (miscRNA)	54	58	65	67	67	67
MGEs
Prophage	Remnant of φAH18-Pro1	φ VB88-Pro1	φ AK9-Pro1	φ VB16-Pro1	φ AI14-Pro1	φ AP20-Pro1
				φ VB16-Pro2
		φ VB88-Pro2
				φ VB16-Pro3
Pathogenicity islands	-	-	-	-	SpPI-181	SpPI-181
Antimicrobial resistance genes and location
SCCmec	ψSCCmec57395	ψSCCmec57395	SCCmecAI16-SCCczrAI16-CI	SCCmecAI16-SCCczrAI16-CI	SCCmecV(T)	SCCmecV(T)
Transposon (Tn)
• Tn5405-like	aadE-sat4-aphA-3 and erm(B)	aadE-sat4-aphA-3 and erm(B)	aadE-sat4-aphA-3, erm(B), and dfrG	aadE-sat4-aphA-3, erm(B), and dfrG	aadE-sat4-aphA-3, erm(B), and dfrG	aadE-sat4-aphA-3, erm(B), and dfrG
• Tn552	blaZ, blaR1, and blaI-1	blaZ, blaR1, and blaI-1	blaZ, blaR1, and blaI-1	blaZ, blaR1, and blaI-1	blaZ, blaR1, and blaI-1	blaZ, blaR1, and blaI-1
• Tn916	tet(M)	tet(M)	tet(M)	tet(M)	tet(M)	tet(M)
Integrated plasmid	cat	cat	-	-	-	-
Insertion sequence (IS)
• IS256	aph(2’’)-Ia	aph(2’’)-Ia	aph(2’’)-Ia	aph(2’’)-Ia	aph(2’’)-Ia	aph(2’’)-Ia
CWA protein genes
• spsA-spsR (18 genes)	spsA-spsE, spsG-spsR (17)	spsA-spsE, spsG-spsO, spsR (15)	spsA-spsE, spsG-spsN, spsP-spsR (16)	spsA-spsE, spsG-spsN, spsP-spsR (16)	spsA-spsE, spsG-spsN, spsR (14)	spsA-spsE, spsG-spsN, spsR (14)
CRISPR subtype	-	-	Type IIC	Type IIC	Type IIIA	Type IIIA