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. 2021 Jul 12;17(7):e1009656. doi: 10.1371/journal.pgen.1009656

Fig 3. Characterization of plasmids encoding an essential gene.

Fig 3

A, Genomic map of unstable pGroE and stable pGroE-S plasmids that were introduced into E. coli MG1655 (plasmid is non-essential) and E. coli MGM100 (plasmid is essential) hosts. B, Plasmid loss frequency of plasmids lacking (-) groE or encoding (+) groE in their genome in the host E. coli MG1655 (H0: L+>L-, P = 0.0247 using Wilcoxon test n = 6) C, Plasmid loss frequency of pGroE-S (stable) and pGroE (unstable) after incubation at 37°C or at 42°C (H0: L42>L37, P = 0.0091 using Wilcoxon test, n = 6). D, Growth rates of host strains E. coli MG1655 and E. coli MGM100 carrying pGroE (unstable) or pGroE-S (stable) after growth at 37°C and 42°C (H0: Gr42>Gr37, P = 0.051 using Wilcoxon test, n = 9).