FIGURE 2.

Candida glabrata strain ATCC90030 was more tolerant to caspofungin than strain ATCC2001HTL in the mouse model of gastrointestinal (GI) colonization, leading to a higher rate of rebound and emergence of echinocandin resistant fks-mutants. Six mice per group were colonized with the indicated C. glabrata strains and exposed to daily high-dose caspofungin treatment starting at day 3 post-colonization. CFU per gram of stool were plotted for each day of sample collection, and frequencies for rebound and fks mutations are indicated in pie charts. Zero and three fks-mutants were obtained in ATCC2001HTL and ATCC90030 background, respectively. All obtained mutations were in the FKS2 gene, and the specific amino acid changes are listed in the pie chart.