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. 2021 Jul 12;10:e67753. doi: 10.7554/eLife.67753

Figure 1. The loss of E2F in fat body does not impair muscle formation.

(A) Confocal Z-stack-projected images of third instar larval body wall muscles ventral longitudinal 3 (VL3) (marked with white arrowhead) and ventral longitudinal 4 (VL4) from the segment A4 stained with Phalloidin, Dp::GFP, and DAPI. Anterior is to the left. Scale: 100 μm. (B) Box plot showing quantification of VL3 muscle area. Whiskers are min to max values, Kruskal-Wallis test followed by Dunn’s multiple comparisons test, **p<0.0001. n=9–12 animals per genotype. Three independent experiments were done. Full genotypes are (A–B) cg-GAL4/UAS-RFP,Dp[GFP], cg-GAL4/Dp[GFP],UAS-Dp[GD4444]-RNAi, UAS-RFP,Dp[GFP];Mef2-GAL4, and Dp[GFP],UAS-Dp[GD4444]-RNAi,Mef2-GAL4.

Figure 1—source data 1. Area measurements of the VL3 muscle (μm2) at the third instarlarva and the statistical analysis.

Figure 1.

Figure 1—figure supplement 1. E2F in muscles and fat body is required for animal development.

Figure 1—figure supplement 1.

Animal viability assessed by quantifying the percentage of animals at each developmental stage (adult, pharate, and early pupa). Data are presented as stacked bar plot, mean ± SEM, n=2 replicates per genotype, repeated as N=5–6 independent experiments. Full genotypes are cg-GAL4,UAS-mCherry-RNAi, cg-GAL4/UAS-Dp[GD4444]-RNAi, UAS-GC3Ai; Lpp-GAL4/UAS-mCherry-RNAi, UAS-GC3Ai/UAS-Dp[GD4444]-RNAi; Lpp-GAL4, Mef2-GAL4/UAS-mCherry-RNAi, and UAS-Dp[GD4444]-RNAi,Mef2-GAL4.