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. 2021 Jul 12;10:e67753. doi: 10.7554/eLife.67753

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© 2021, Zappia et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A) Confocal single plane images of third instar larval fat bodies stained with phalloidin, 4,6-diamidino-2-phenylindole (DAPI), and mouse anti-Dp antibody (Yun). White arrowheads point to newly formed binucleate cells. (B) Quantification of the percentage of binucleated cells in fat body. Date are presented as mean ± SD, n=415 cells for Mef2>Dp-RNAi, n=405 cells for cg>Dp-RNAi, and n=300 cells for each Mef2>Luc-RNAi and cg>Luc-RNAi, which did not show binucleates. Kruskal-Wallis test followed by Dunn’s multiple comparisons test, p<0.0001. Experiment was repeated two times. One representative experiment is shown. (C) Confocal single plane images of third instar larval fat bodies stained with Rad50, phalloidin, and DAPI. Scale: 100 μm. Full genotypes are (A) cg-GAL4;UAS-mCherry-RNAi, cg-GAL4/UAS-Dp[GD4444]-RNAi, Mef2-GAL4/ UAS-luciferase[JF01355]-RNAi, and UAS-Dp[GD4444]-RNAi; Mef2-GAL4, (B) cg-GAL4/UAS-RFP,Dp[GFP], cg-GAL4/Dp[GFP],UAS-Dp[GD4444]-RNAi, UAS-RFP,Dp[GFP];Mef2-GAL4, and Dp[GFP],UAS-Dp[GD4444]-RNAi,Mef2-GAL4, and (C) cg-GAL4,UAS-mRFP;UAS-luciferase[JF01355]-RNAi, cg-GAL4,UAS-mRFP/UAS-Dp[GD4444]-RNAi, Mef2-GAL4/UAS-luciferase[JF01355]-RNAi, and UAS-Dp[GD4444]-RNAi;Mef2-GAL4.

Figure 2—figure supplement 1. — (A) Confocal single plane images of third instar larval fat bodies stained with phalloidin, 4,6-diamidino-2-phenylindole (DAPI), and mouse anti-Dp antibody (Yun). White arrowheads point to newly formed binucleate cells. (B) Quantification of the percentage of binucleated cells in fat body. Date are presented as mean ± SD, n=415 cells for Mef2>Dp-RNAi, n=405 cells for cg>Dp-RNAi, and n=300 cells for each Mef2>Luc-RNAi and cg>Luc-RNAi, which did not show binucleates. Kruskal-Wallis test followed by Dunn’s multiple comparisons test, p<0.0001. Experiment was repeated two times. One representative experiment is shown. (C) Confocal single plane images of third instar larval fat bodies stained with Rad50, phalloidin, and DAPI. Scale: 100 μm. Full genotypes are (A) cg-GAL4;UAS-mCherry-RNAi, cg-GAL4/UAS-Dp[GD4444]-RNAi, Mef2-GAL4/ UAS-luciferase[JF01355]-RNAi, and UAS-Dp[GD4444]-RNAi; Mef2-GAL4, (B) cg-GAL4/UAS-RFP,Dp[GFP], cg-GAL4/Dp[GFP],UAS-Dp[GD4444]-RNAi, UAS-RFP,Dp[GFP];Mef2-GAL4, and Dp[GFP],UAS-Dp[GD4444]-RNAi,Mef2-GAL4, and (C) cg-GAL4,UAS-mRFP;UAS-luciferase[JF01355]-RNAi, cg-GAL4,UAS-mRFP/UAS-Dp[GD4444]-RNAi, Mef2-GAL4/UAS-luciferase[JF01355]-RNAi, and UAS-Dp[GD4444]-RNAi;Mef2-GAL4.