Figure 4. Restoring E2F/Dp in muscles suppresses defect in Dp-deficient fat body.

(A) Confocal single plane images of third instar larval fat bodies immunostained with anti-Dp antibody (212), phalloidin, and 4,6-diamidino-2-phenylindole (DAPI). Note that Dp^-/-;Mef2>Dp rescued animals do not show binucleated cells, but many nuclei are fragmented and decondensed. Scale: 50 μm. (B) Quantification of percentage of binucleated cells as in A. Data presented as bar plot showing mean ± SD, Kruskal-Wallis test followed by Dunn’s multiple comparisons test, ***p<0.0001, n=16 animals per genotype, two independent experiments were done. At least 606 cells were scored. (C) Confocal Z-stack-projected images of third instar larval body wall muscles ventral longitudinal 3 (VL3) (marked with white arrowhead) and ventral longitudinal 4 (VL4) from the segment A4 immunostained with rabbit anti-Dp antibody (212), phalloidin, and DAPI. Anterior is to the left. Scale: 100 μm. (D) Quantification of VL3 muscle area as in C. Data presented as box plot, whiskers min to max values, Kruskal-Wallis test followed by Dunn’s multiple comparisons test, ***p<0.0001, n=15 animals per genotype, except n=11 for Dp-/-, two independent experiments were done. Full genotypes are Dp⁺/Dp^a3,cg-GAL4;UAS-Dp, Dp^Exel7124/Dp^a3,cg-GAL4, Dp^Exel7124/Dp^a3,cg-GAL4;UAS-Dp, Dp^Exel7124/Dp^a3; Mef2-GAL4/UAS-Dp.