Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene | GIPR_human | NCBI | NM_000164.4 | |
| Strain, strain background(Escherichia coli) | BL21 (DE3) | TIANGEN | Cat # CB105 | |
| Cell line (Homo sapiens) | HEK 293T | ATCC | Cat # CRL-3216 | |
| Cell line (hamster) | CHO-K1 | ATCC | Cat # CCL-61 | |
| Cell line (insect) | Sf9 | Invitrogen | N/A | |
| Cell line (insect) | High-Five insect cells | ThermoFisher Scientific | Cat # B85502 | |
| Recombinant DNA reagent |
pFastBac-HA-BRIL-TEV-2GSA-GIPR(22-421)T345F-15AA-LgBiT-TEV-OMBP-MBP | This paper | N/A | |
| Recombinant DNA reagent |
pFastBac-HA-BRIL-TEV-2GSA-GIPR(22-421)−15AA-LgBiT-TEV-OMBP-MBP | This paper | N/A | |
| Recombinant DNA reagent |
pFastBac-DNGαs | This paper | N/A | |
| Recombinant DNA reagent |
pFastBac-Gβ1-peptide 86 | https://doi.org/10.1038/s41422-020-00442-0 | N/A | |
| Recombinant DNA reagent |
pFastBac-Gγ2 | https://doi.org/10.1038/s41422-020-00442-0 | N/A | |
| Recombinant DNA reagent |
PMESy4-Nb35 | https://doi.org/10.1016/j.molcel.2020.01.013 | N/A | |
| Recombinant DNA reagent |
pcDNA3.1-GIPR (WT and mutants)−3Flag | This paper | N/A | |
| Peptide, recombinant protein | GIP1-42 | GenScript | N/A | |
| Chemical compound, drug | Protease Inhibitor Cocktail, EDTA-Free | TragetMol | Cat # C0001 | |
| Chemical compound, drug | Apyrase | Sigma-Aldrich (Merck) | Cat # A6132 | |
| Chemical compound, drug | TCEP | Sigma-Aldrich (Merck) | Cat # C4706 | |
| Chemical compound, drug | Lauryl maltose neopentylglycol (LMNG) | Anatrace | Cat # NG310 | |
| Chemical compound, drug | Cholesterol hemisuccinate (CHS) | Anatrace | Cat # CH210 | |
| Chemical compound, drug | Glyco-diosgenin (GDN) | Anatrace | Cat # GDN101 | |
| Chemical compound, drug | Amylose resin | NEB | Cat # E8021L | |
| Chemical compound, drug | ESF 921 culture medium | Expression Systems | Cat # 96-00-01 | |
| Chemical compound, drug | Fetal bovine serum (FBS) | Gibco | Cat # 10099–141 | |
| Chemical compound, drug | DMEM | Gibco | Cat # 12430–054 | |
| Chemical compound, drug | X-tremeGHNE HP DNA Transfection Reagent | Sigma-Aldrich (Roche) | Cat # 6366236001 | |
| Chemical compound, drug | Digitonin | Biosynth | Cat # D-3203 | |
| Chemical compound, drug | Salt active nuclease | Sigma-Aldrich | Cat # SRE0015-5KU | |
| Chemical compound, drug | Sodium pyruvate | Gibco | Cat # 11360–0’70 | |
| Chemical compound, drug | Lipofectamine 2000 transfection reagent | Invitrogen | Cat # 11668–019 | |
| Chemical compound, drug | 125I-GIP | PerkinElmer | Cat # NEX402010UC | |
| Chemical compound, drug | BSA | ABCONE | Cat # A23088-100G | |
| Antibody | Anti-Flag primary antibody | Sigma-Aldrich | Cat # F3165 | |
| Antibody | Anti-mouse Alexa Fluor 488 conjugated secondary antibody | Invitrogen | Cat # A-21202 | |
| Commercial assay, kit | LANCE Ultra cAMP kit | PerkinElmer | Cat # 2675984 | |
| Software, algorithm | MotionCor2.1 | doi:10.1126/science.aav7942 | N/A | https://msg.ucsf.edu/em/software/motioncor2.html |
| Software, algorithm | Gctf v1.06 | https://doi.org/10.1016/j.jsb.2015.11.003 | N/A | https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/ |
| Software, algorithm | RELION-3.0-beta2 | https://doi.org/10.1016/j.jsb.2012.09.006 | N/A | https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Download_%26_install |
| Software, algorithm | COOT | https://doi.org/10.1107/S0907444904019158 | N/A | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Software, algorithm | Phenix | https://doi.org/10.1107/S0907444909052925 | N/A | http://www.phenix-online.org/ |
| Software, algorithm | Chimera | https://doi.org/10.1002/jcc.20084 | N/A | https://www.cgl.ucsf.edu/chimera/ |
| Software, algorithm | PyMOL | Schrödinger | N/A | https://pymol.org/2/ |
| Software, algorithm | GraphPad Prism v7.0 | GraphPad Software | N/A | https://www.graphpad.com/ |
| Software, algorithm | FreeSASA | doi:10.12688/f1000research.7931.1 | N/A | http://freesasa.github.io/ |
| Software, algorithm | Gromacs 2018.5 | doi:10.1016/j.softx.2015.06.001 | N/A | https://manual.gromacs.org/2018.5/download.html |
| Software, algorithm | Protein Preparation Wizard |
Schrödinger | N/A | https://www.schrodinger.com/products/protein-preparation-wizard |
| Software, algorithm | CHARMM-GUI Membrane Builder |
https://doi.org/10.1002/jcc.23702 | N/A | https://charmm-gui.org/ |
| Software, algorithm | CHARMM36-CAMP | https://doi.org/10.1021/ct200328p | N/A | |
| Software, algorithm | LINCS algorithm | https://doi.org/10.1021/ct700200b | N/A | |
| Software, algorithm | Semi-isotropic Parrinello-Rahman barostat | https://doi.org/10.1016/0022-3093(93)90111-A | N/A |