Table 1. Cryo-EM data collection, refinement, and validation statistics.
| GIP–GIPR–Gs–Nb35 complex | |
|---|---|
| Data collection and processing | |
| Magnification | 46,685 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 80 |
| Defocus range (μm) | −1.2 to −2.2 |
| Pixel size (Å) | 1.071 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 4,895,399 |
| Final particle images (no.) | 295,021 |
| Map resolution (Å) FSC threshold |
2.9 0.143 |
| Map resolution range (Å) | 2.7–5.0 |
| Refinement | |
| Initial model used (PDB code) | PDB codes 6WPW and 2QKH |
| Model resolution (Å) FSC threshold |
2.9 0.5 |
| Model resolution range (Å) | 2.7–5.0 |
| Map sharpening B factor (Å2) | −86.3 |
| Model composition Non-hydrogen atoms Protein residues Lipids |
9409 1156 6 |
| B factors (Å2) Protein Ligand Lipids |
133 143 121 |
| R.m.s. deviations Bond lengths (Å) Bond angles (Å) |
0.005 1.036 |
| Validation MolProbity score Clash score Poor rotamers (%) |
1.21 4.23 0.00 |
| Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
98.15 1.85 0.00 |