Skip to main content
. 2021 May 6;40(29):4746–4758. doi: 10.1038/s41388-021-01800-x

Fig. 2. Effect of AT-related STLs on cell viability and induction of apoptosis and differentiation of myeloid leukemia cell lines.

Fig. 2

A NB4, HL60, and Kasumi-1 cells were treated with the indicated concentrations of AT, AMA, or AIB for 48 h. Cell viability was analyzed by a MTT assay. B NB4, HL60, and Kasumi-1 cells were treated for 2 days with the indicated concentrations of AT or AIB. The cells were then stained with antibodies against CD11b or CD14 and analyzed by flow cytometry. Bars indicate the percentage of positive cells with standard deviations. C Cells treated as in (B) were stained with annexin V-FITC (A) and propidium iodide (PI) and analyzed by flow cytometry. A-/PI-: living cells; A + /PI- and A + /PI + : early and late apoptotic cells; A-/PI + : necrotic cells. Bar charts indicate the percentage (with standard deviations) of living and apoptotic/necrotic cells of the whole cell population. The bottom shows western blot analyses of aliquots of the cells illustrating the expression of MYB, C/EBPβ, and β-actin.