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. 2021 May 6;40(29):4746–4758. doi: 10.1038/s41388-021-01800-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Decreased MYC and GFI1 expression in U937 cells after treatment for 2 and 4 h with AT (1 μM) or HA (0.5 μM). Statistical significance (*p < 0.05, ***p < 0.001, Student’s t test). B U937 cells expressing ectopic wild-type GFI1 (U937/MIC-GFI1), an inactive mutant of GFI1 (U937/MIC-GFI1-P2A) or no GFI1(U937/MIC) were analyzed by flow cytometry for CD11b expression. The bars indicate the percentage of CD11b positive cells after treatment for 4 days with DMSO, 0.5 μM HA or 2 μM AMA. Statistical significance was determined with one-way ANOVA with Tukey correction (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). C Schematic illustration of GFI1 transcript variants and nucleotide sequence of the GFI1 variant 3 promoter region. Bold underlined letters correspond to the 5’ end of human GFI1 variant 3 mRNA. Sequences in red and blue mark MYB and C/EBPβ binding sites, respectively. Conservation of these sequences in the mouse genome is indicated below. D Comparison of the luciferase activity of the cloned GFI promoter and the promoter-less luciferase vector. E, F Reporter assays of the human GFI1 promoter in the presence of the indicated combinations of expression vectors for MYB-Δ3, C/EBPβ, and p300. In panel E, Western blotting (bottom) confirms the expression of the indicated proteins. Reporter assays in panel (F) were performed without or with 1 μM HA. Asterisks in panels (E) and (F) indicate statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Student’s t test; NS, non-specific).