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. 2021 Jun 18;40(29):4759–4769. doi: 10.1038/s41388-021-01876-5

Fig. 5. Chromatin-opening ability of EWS/FLI is unaltered by deletions flanking the FLI DNA-binding domain.

Fig. 5

A All EWS/FLI-bound loci in A673 cells (determined by CUT&RUN of knock-down/rescue cells expressing full-length EWS/FLI [EF]) were compared to loci harboring ATAC signal peaks and shown in graphical format (ATAC performed on N = 2 biological replicates each). There were 12 482 EF-bound peaks with ATAC signal and 696 EF-bound peaks without ATAC signal. B Venn diagram analysis of regions bound by EF DBD+ and/or EF DBD that also had overlapping ATAC signals. C–D Representative tracks of RNA-sequencing, CUT&RUN genomic localization, and ATAC-sequencing signals for the indicated knock-down/rescue A673 cells (EF KD = iEF + EF; EF DBD = iEF + EF DBD; EF DBD + = iEF + EF DBD+). Scales to view tracks were kept consistent across sequencing type in each panel and are represented on the right. Representative genes PPP1R1A (C) and STEAP1 (D) are regulated by EF DBD+ but not EF DBD (adjusted p-value < 0.05) and overlapping CUT&RUN and ATAC-sequencing peaks are highlighted.