Table 3.
Catalytic efficiencies of BdPTE mutants towards G-type nerve agents
| kcat/KM (× 106 M−1 min−1) | BdPTE-1 | BdPTE-2b | BdPTE-3 | BdPTE-4 | BdPTE-5b | BdPTE-6 | BdPTE-7 |
|---|---|---|---|---|---|---|---|
| GA | 225 ± 11.6 | 647 ± 11.6 | 582 ± 8.29 | 313 ± 3.99 | 136 ± 0.10 | 133 ± 3.19 | 380 ± 6.74 |
| GB | 106 ± 0.48 | 165 ± 2.70a | 200 ± 3.68a | 142 ± 1.93 | 52.0 ± 0.82 | 71.1 ± 1.28 | 205 ± 11.8 |
| GF | 2.14 ± 0.05 | 5.31 ± 0.21a | 6.37 ± 0.21a | 5.03 ± 0.04 | 10.2 ± 1.49 | 9.72 ± 0.31 | 13.6 ± 0.23 |
| GD | 4.51 ± 0.08 | 5.06 ± 0.29 | 6.80 ± 0.11 | 4.87 ± 0.08 | 1.99 ± 0.01 | 3.23 ± 0.14 | 7.18 ± 0.05 |